An Integrated In Vitro Imaging Platform for Characterizing Filarial Parasite Behavior within a Multicellular Microenvironment
Identifieur interne : 002D65 ( Main/Exploration ); précédent : 002D64; suivant : 002D66An Integrated In Vitro Imaging Platform for Characterizing Filarial Parasite Behavior within a Multicellular Microenvironment
Auteurs : Timothy Kassis [États-Unis] ; Henry M. Skelton [États-Unis] ; Iris M. Lu [États-Unis] ; Andrew R. Moorhead [États-Unis] ; J. Brandon Dixon [États-Unis]Source :
- PLoS Neglected Tropical Diseases [ 1935-2727 ] ; 2014.
Descripteurs français
- KwdFr :
- Algorithmes, Animaux, Brugia malayi (physiologie), Cellules cultivées, Cellules endothéliales (cytologie), Fibroblastes (cytologie), Filariose lymphatique (parasitologie), Humains, Microenvironnement cellulaire (physiologie), Parasitologie (), Parasitologie (instrumentation), Système lymphatique (cytologie), Traitement d'image par ordinateur (), Traitement d'image par ordinateur (instrumentation), Vidéomicroscopie ().
- MESH :
- cytologie : Cellules endothéliales, Fibroblastes, Système lymphatique.
- parasitologie : Filariose lymphatique.
- physiologie : Brugia malayi, Microenvironnement cellulaire.
- instrumentation : Algorithmes, Animaux, Cellules cultivées, Humains, Parasitologie, Traitement d'image par ordinateur, Vidéomicroscopie.
English descriptors
- KwdEn :
- Algorithms, Animals, Brugia malayi (physiology), Cells, Cultured, Cellular Microenvironment (physiology), Elephantiasis, Filarial (parasitology), Endothelial Cells (cytology), Fibroblasts (cytology), Humans, Image Processing, Computer-Assisted (instrumentation), Image Processing, Computer-Assisted (methods), Lymphatic System (cytology), Microscopy, Video (methods), Parasitology (instrumentation), Parasitology (methods).
- MESH :
- cytology : Endothelial Cells, Fibroblasts, Lymphatic System.
- instrumentation : Image Processing, Computer-Assisted, Parasitology.
- methods : Image Processing, Computer-Assisted, Microscopy, Video, Parasitology.
- parasitology : Elephantiasis, Filarial.
- physiology : Brugia malayi, Cellular Microenvironment.
- Algorithms, Animals, Cells, Cultured, Humans.
Abstract
Lymphatic Filariasis, a Neglected Tropical Disease, is caused by thread-like parasitic worms, including
Url:
DOI: 10.1371/journal.pntd.0003305
PubMed: 25412444
PubMed Central: 4238983
Affiliations:
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Le document en format XML
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Petit Institute for Bioengineering & Bioscience, Georgia Institute of Technology, Atlanta, Georgia, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Parker H. 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Petit Institute for Bioengineering & Bioscience, Georgia Institute of Technology, Atlanta, Georgia, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Parker H. Petit Institute for Bioengineering & Bioscience, Georgia Institute of Technology, Atlanta, Georgia</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="aff3"><addr-line>Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Lu, Iris M" sort="Lu, Iris M" uniqKey="Lu I" first="Iris M." last="Lu">Iris M. Lu</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Parker H. Petit Institute for Bioengineering & Bioscience, Georgia Institute of Technology, Atlanta, Georgia, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Parker H. Petit Institute for Bioengineering & Bioscience, Georgia Institute of Technology, Atlanta, Georgia</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="aff4"><addr-line>George W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, Georgia, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>George W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, Georgia</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Moorhead, Andrew R" sort="Moorhead, Andrew R" uniqKey="Moorhead A" first="Andrew R." last="Moorhead">Andrew R. Moorhead</name><affiliation wicri:level="2"><nlm:aff id="aff5"><addr-line>Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, Georgia, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, Georgia</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Dixon, J Brandon" sort="Dixon, J Brandon" uniqKey="Dixon J" first="J. Brandon" last="Dixon">J. Brandon Dixon</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Parker H. Petit Institute for Bioengineering & Bioscience, Georgia Institute of Technology, Atlanta, Georgia, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Parker H. Petit Institute for Bioengineering & Bioscience, Georgia Institute of Technology, Atlanta, Georgia</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="aff4"><addr-line>George W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, Georgia, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>George W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, Georgia</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author></analytic><series><title level="j">PLoS Neglected Tropical Diseases</title><idno type="ISSN">1935-2727</idno><idno type="eISSN">1935-2735</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Algorithms</term><term>Animals</term><term>Brugia malayi (physiology)</term><term>Cells, Cultured</term><term>Cellular Microenvironment (physiology)</term><term>Elephantiasis, Filarial (parasitology)</term><term>Endothelial Cells (cytology)</term><term>Fibroblasts (cytology)</term><term>Humans</term><term>Image Processing, Computer-Assisted (instrumentation)</term><term>Image Processing, Computer-Assisted (methods)</term><term>Lymphatic System (cytology)</term><term>Microscopy, Video (methods)</term><term>Parasitology (instrumentation)</term><term>Parasitology (methods)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Algorithmes</term><term>Animaux</term><term>Brugia malayi (physiologie)</term><term>Cellules cultivées</term><term>Cellules endothéliales (cytologie)</term><term>Fibroblastes (cytologie)</term><term>Filariose lymphatique (parasitologie)</term><term>Humains</term><term>Microenvironnement cellulaire (physiologie)</term><term>Parasitologie ()</term><term>Parasitologie (instrumentation)</term><term>Système lymphatique (cytologie)</term><term>Traitement d'image par ordinateur ()</term><term>Traitement d'image par ordinateur (instrumentation)</term><term>Vidéomicroscopie ()</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Cellules endothéliales</term><term>Fibroblastes</term><term>Système lymphatique</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Endothelial Cells</term><term>Fibroblasts</term><term>Lymphatic System</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Image Processing, Computer-Assisted</term><term>Parasitology</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Image Processing, Computer-Assisted</term><term>Microscopy, Video</term><term>Parasitology</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Brugia malayi</term><term>Microenvironnement cellulaire</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Brugia malayi</term><term>Cellular Microenvironment</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Algorithms</term><term>Animals</term><term>Cells, Cultured</term><term>Humans</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="fr"><term>Algorithmes</term><term>Animaux</term><term>Cellules cultivées</term><term>Humains</term><term>Parasitologie</term><term>Traitement d'image par ordinateur</term><term>Vidéomicroscopie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Lymphatic Filariasis, a Neglected Tropical Disease, is caused by thread-like parasitic worms, including <italic>B. malayi</italic>, which migrate to the human lymphatic system following transmission. The parasites reside in collecting lymphatic vessels and lymph nodes for years, often resulting in lymphedema, elephantiasis or hydrocele. The mechanisms driving worm migration and retention within the lymphatics are currently unknown. We have developed an integrated <italic>in vitro</italic> imaging platform capable of quantifying <italic>B. malayi</italic> migration and behavior in a multicellular microenvironment relevant to the initial site of worm injection by incorporating the worm in a Polydimethylsiloxane (PDMS) microchannel in the presence of human dermal lymphatic endothelial cells (LECs) and human dermal fibroblasts (HDFs). The platform utilizes a motorized controllable microscope with CO<sub>2</sub> and temperature regulation to allow for worm tracking experiments with high resolution over large length and time scales. Using post-acquisition algorithms, we quantified four parameters: 1) speed, 2) thrashing intensity, 3) percentage of time spent in a given cell region and 4) persistence ratio. We demonstrated the utility of our system by quantifying these parameters for L3 <italic>B. malayi</italic> in the presence of LECs and HDFs. Speed and thrashing increased in the presence of both cell types and were altered within minutes upon exposure to the anthelmintic drug, tetramisole. The worms displayed no targeted migration towards either cell type for the time course of this study (3 hours). When cells were not present in the chamber, worm thrashing correlated directly with worm speed. However, this correlation was lost in the presence of cells. The described platform provides the ability to further study <italic>B. malayi</italic> migration and behavior.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Karpanen, T" uniqKey="Karpanen T">T Karpanen</name></author><author><name sortKey="Alitalo, K" uniqKey="Alitalo K">K Alitalo</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Kusaba, T" uniqKey="Kusaba T">T Kusaba</name></author><author><name sortKey="Fujimaki, Y" uniqKey="Fujimaki Y">Y Fujimaki</name></author><author><name sortKey="Vincent, Al" uniqKey="Vincent A">AL Vincent</name></author><author><name sortKey="Aoki, Y" uniqKey="Aoki Y">Y Aoki</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pani, S" uniqKey="Pani S">S Pani</name></author><author><name sortKey="Srividya, A" uniqKey="Srividya A">A 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Laney</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Géorgie (États-Unis)</li></region></list><tree><country name="États-Unis"><region name="Géorgie (États-Unis)"><name sortKey="Kassis, Timothy" sort="Kassis, Timothy" uniqKey="Kassis T" first="Timothy" last="Kassis">Timothy Kassis</name></region><name sortKey="Dixon, J Brandon" sort="Dixon, J Brandon" uniqKey="Dixon J" first="J. Brandon" last="Dixon">J. Brandon Dixon</name><name sortKey="Dixon, J Brandon" sort="Dixon, J Brandon" uniqKey="Dixon J" first="J. Brandon" last="Dixon">J. Brandon Dixon</name><name sortKey="Kassis, Timothy" sort="Kassis, Timothy" uniqKey="Kassis T" first="Timothy" last="Kassis">Timothy Kassis</name><name sortKey="Lu, Iris M" sort="Lu, Iris M" uniqKey="Lu I" first="Iris M." last="Lu">Iris M. Lu</name><name sortKey="Lu, Iris M" sort="Lu, Iris M" uniqKey="Lu I" first="Iris M." last="Lu">Iris M. Lu</name><name sortKey="Moorhead, Andrew R" sort="Moorhead, Andrew R" uniqKey="Moorhead A" first="Andrew R." last="Moorhead">Andrew R. Moorhead</name><name sortKey="Skelton, Henry M" sort="Skelton, Henry M" uniqKey="Skelton H" first="Henry M." last="Skelton">Henry M. Skelton</name><name sortKey="Skelton, Henry M" sort="Skelton, Henry M" uniqKey="Skelton H" first="Henry M." last="Skelton">Henry M. Skelton</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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